Multiplexing protein and gene level measurements on a single. A multiplex branched dna assay for parallel quantitative. Quantigene singleplex assay thermo fisher scientific. Multiplexed quantitative gene expression protocol using branched dna technology from the panomics solutions from affymetrix.
Quantigene plex assays provide a fast and highthroughput solution for multiplexed gene expression quantitation, allowing the simultaneous measurement of up to 80 genes of interest in a single well of a 96 or 384well plate. We investigated the potential of quantigene plex qgpbranched. If a paper cites a quantigene plex dna reagent system product and is published in a research journal, the lead authors may receive a travel stipend for use at a. The multiplex bdna assays were run as recommended in the quantigene plex 2. Quantigene plex represents a promising diagnostic tool for cellof. Contents and storage kit components have a shelf life of 12 months from the date of receipt. Clear instructions on how to assess the data quality and tools to perform this analysis in high throughput mode are, however, currently lacking. Maqc study data generation plan genospectra quantigene.
About this user manual this manual is for anyone who has purchased a quanti gene plex assay kit and quantigene plex set and intends to perform the quantigene plex assay for any of the following sample types. Automated washing of magnetic bead quantigene plex 2. The bdna assay is a hybridizationbased method of targetspecific rna quantitation. Experiments were performed according to manufacturers instructions. User manual quantigene plex assay luminex 100, luminex 200, flexmap 3d, magpix, bio plex luminex corporation vortex mixer major laboratory supplier mls email this biorad powerpac 200 powerpac 300 manual bio plex multiplex system purchasing and service programs. Optional for 4 plex assay quantigene viewrna ish cell accessory kit optional to perform a basic 1 to 3 plex viewrna ish assay, the quantigene viewrna ish cell assay kit and quantigene viewrna probe sets are required.
Genospectra quantigene study plan and sop for maqc 1 q. Panomics package insert solutions thermo fisher scientific. Quantigene is a registered trademark exclusively licensed to affymetrix, inc. The quantigene plex assay utilizes xmap luminex beads for multiplexing of 3 to 80 rna targets followed by branched dnabased signal amplification. The quantigene plex assay is hybridizationbased and incorporates branched dna bdna technology, which uses signal. All other trademarks are the property of their respective owners. Probe design software 22 was used to generate probe sets for singleplex or multiplex. Plex sizes between 65 and 80 cultured cells blood samples whole blood, paxgene blood, or dried blood spots. Qs0110 qs0111 qs0112 kit size 2plate 10plate 5 x 10plates storage component quantity quantity quantity lysis mixture. Watch this video to learn how the quantigene plex assay works. The assay is performed in a 96well plate and signal is detected using a luminex instrument.
User manual thermo fisher scientific this manual is for anyone who has purchased a quantigene plex assay kit and 1 we recommend storing in an enzyme storage box, such as the neb cool. Protein procartaplex assay and gene expression quantigene plex assay levels. Refer to the quantigene and quantigene plex user manual for more information about running the assays. View and download jacobsen gplex iii safety and operation manual online.
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